- Author
- Title
- Measuring microvascular and mitochondrial oxygen tension: novel techniques for studying tissue oxygenation
- Supervisors
-
C. Ince
- Co-supervisors
-
C.J. Zuurbier
- Award date
- 1 March 2011
- Number of pages
- 156
- ISBN
- 9789461081360
- Document type
- PhD thesis
- Faculty
- Faculty of Medicine (AMC-UvA)
- Abstract
-
This thesis describes the implementation of novel approaches for measuring tissue oxygenation based on oxygen-dependent quenching of the triplet-state lifetime of porphyrins. Ultimately we created a tool for assessment of mitochondrial oxygen tension in vivo. The first measurements indicate much higher mitoPO2 values than generally expected. These findings come in an era of new insights in cellular oxygen sensing and oxygen-dependence of gene expression and metabolism. The described methods are expected to be a valuable addition to the arsenal of tools available to scientist for unraveling the mechanisms of oxygen delivery and consumption under various pathophysiological circumstances. Oxygen is again important, as it has always been
- Note
- Author's name on the cover: Bert Mik.
- Persistent Identifier
- https://hdl.handle.net/11245/1.366201
- Downloads
-
Thesis
Cover
Title pages
Table of contents
Chapter 1: General introduction
Chapter 2: Excitation pulse deconvolution in luminescence lifetime analysis for oxygen measurements in vivo
Chapter 3: Quantitative determination of localized tissue oxygen concentration in vivo by two-photon excitation phosphorescence lifetime measurements
Chapter 4: Dual-wavelength phosphorimetry for determination of cortical and subcortical microvascular oxygenation in rat kidney
Chapter 5: Monitoring of renal venous PO2 and kidney oxygen consumption in rats by a near-infrared phosphorescence lifetime technique
Chapter 6: Mitochondrial PO2 measured by delayed fluorescence of endogenous protoporphyrin IX
Chapter 7: In vivo mitochondrial oxygen tension measured by a delayed fluorescence lifetime technique
Chapter 8: Mitochondrial oxygen tension within the heart
Chapter 9: Summary and conclusions
Samenvatting en conclusies
Dankwoord
List of publications
Curriculum vitae
Stellingen
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